Conventional end-point measurements can be misleading because the fluorescence intensity is influenced by both the peptide’s binding characteristics, as well as its synthesis yield.
As a result, peptides with different KD values can produce similar signals under saturated conditions, while peptides with similar affinities may yield different signals.
This makes it impossible to accurately rank or compare binding strengths without proper affinity measurements.
To overcome the limitations of end-point measurements, we perform a concentration series of the analyte and record binding signals across multiple concentrations.
This allows for precise affinity ranking, even across peptides with varying synthesis yields or binding kinetics.
We design and synthesize a high-density peptide library in microarray format.
Your protein is applied in a concentration series, and fluorescence is recorded across all peptide spots.
We fit binding curves for each peptide to estimate KD values and rank peptide–protein interactions.
We eliminate synthesis bias so you can trust the binding data.
We assist you through the whole process, from library generation, through protein labeling, to performing the assay and data analysis.
Estimate up to 200,000 KD‘s in a single assay.
Measure thousands of binding affinities in parallel. Reach out to learn how our solutions can support your research goals.
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